Limited effect of reactive oxygen species on the composition of susceptible essential amino acids in the midguts of Lymantria dispar caterpillars.
Identifieur interne : 002997 ( Main/Exploration ); précédent : 002996; suivant : 002998Limited effect of reactive oxygen species on the composition of susceptible essential amino acids in the midguts of Lymantria dispar caterpillars.
Auteurs : Raymond V. Barbehenn [États-Unis] ; Julie Niewiadomski ; Joseph Kochmanski ; C Peter ConstabelSource :
- Archives of insect biochemistry and physiology [ 1520-6327 ] ; 2012.
Descripteurs français
- KwdFr :
- MESH :
- métabolisme : Acides aminés essentiels, Espèces réactives de l'oxygène, Larve, Papillons de nuit, Tube digestif.
- Acer, Animaux, Arbres, Populus, Quercus.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Amino Acids, Essential, Reactive Oxygen Species.
- metabolism : Gastrointestinal Tract, Larva, Moths.
- Acer, Animals, Populus, Quercus, Trees.
Abstract
The essential amino acids (EAAs) arginine, histidine, lysine, and methionine, as well as cysteine (semiessential), are believed to be susceptible to reactions with reactive oxygen species (ROS) in biological systems. The decreased availability of these EAAs could harm insect nutrition, since several of them can also be limiting for protein synthesis. However, no in vivo studies have quantified the effect of ROS in the midguts of insect herbivores on EAA composition. This study examined the association between elevated levels of ROS in the midgut fluid of Lymantria dispar caterpillars and the compositions of EAAs (protein-bound + protein-free) in their midgut fluid and frass. Contrary to expectation, the compositions of EAAs were not significantly decreased by ROS in midgut fluid ex vivo when incubated with phenolic compounds. Two in vivo comparisons of low- and high-ROS-producing leaves also showed similar results: there were no significant decreases in the compositions of EAAs in the midgut fluids and/or frass of larvae with elevated levels of ROS in their midguts. In addition, waste nitrogen excretion was not significantly increased from larvae on high-ROS treatments, as would be expected if ROS produced unbalanced EAA compositions. These results suggest that L. dispar larvae are able to tolerate elevated levels of ROS in their midguts without nutritionally significant changes in the compositions of susceptible EAAs in their food.
DOI: 10.1002/arch.21065
PubMed: 22961657
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Limited effect of reactive oxygen species on the composition of susceptible essential amino acids in the midguts of Lymantria dispar caterpillars.</title><author><name sortKey="Barbehenn, Raymond V" sort="Barbehenn, Raymond V" uniqKey="Barbehenn R" first="Raymond V" last="Barbehenn">Raymond V. Barbehenn</name><affiliation wicri:level="2"><nlm:affiliation>Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA. rvb@umich.edu</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109</wicri:regionArea><placeName><region type="state">Michigan</region></placeName></affiliation></author><author><name sortKey="Niewiadomski, Julie" sort="Niewiadomski, Julie" uniqKey="Niewiadomski J" first="Julie" last="Niewiadomski">Julie Niewiadomski</name></author><author><name sortKey="Kochmanski, Joseph" sort="Kochmanski, Joseph" uniqKey="Kochmanski J" first="Joseph" last="Kochmanski">Joseph Kochmanski</name></author><author><name sortKey="Constabel, C Peter" sort="Constabel, C Peter" uniqKey="Constabel C" first="C Peter" last="Constabel">C Peter Constabel</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:22961657</idno><idno type="pmid">22961657</idno><idno type="doi">10.1002/arch.21065</idno><idno type="wicri:Area/Main/Corpus">002890</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002890</idno><idno type="wicri:Area/Main/Curation">002890</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">002890</idno><idno type="wicri:Area/Main/Exploration">002890</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Limited effect of reactive oxygen species on the composition of susceptible essential amino acids in the midguts of Lymantria dispar caterpillars.</title><author><name sortKey="Barbehenn, Raymond V" sort="Barbehenn, Raymond V" uniqKey="Barbehenn R" first="Raymond V" last="Barbehenn">Raymond V. Barbehenn</name><affiliation wicri:level="2"><nlm:affiliation>Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA. rvb@umich.edu</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109</wicri:regionArea><placeName><region type="state">Michigan</region></placeName></affiliation></author><author><name sortKey="Niewiadomski, Julie" sort="Niewiadomski, Julie" uniqKey="Niewiadomski J" first="Julie" last="Niewiadomski">Julie Niewiadomski</name></author><author><name sortKey="Kochmanski, Joseph" sort="Kochmanski, Joseph" uniqKey="Kochmanski J" first="Joseph" last="Kochmanski">Joseph Kochmanski</name></author><author><name sortKey="Constabel, C Peter" sort="Constabel, C Peter" uniqKey="Constabel C" first="C Peter" last="Constabel">C Peter Constabel</name></author></analytic><series><title level="j">Archives of insect biochemistry and physiology</title><idno type="eISSN">1520-6327</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acer (MeSH)</term><term>Amino Acids, Essential (metabolism)</term><term>Animals (MeSH)</term><term>Gastrointestinal Tract (metabolism)</term><term>Larva (metabolism)</term><term>Moths (metabolism)</term><term>Populus (MeSH)</term><term>Quercus (MeSH)</term><term>Reactive Oxygen Species (metabolism)</term><term>Trees (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acer (MeSH)</term><term>Acides aminés essentiels (métabolisme)</term><term>Animaux (MeSH)</term><term>Arbres (MeSH)</term><term>Espèces réactives de l'oxygène (métabolisme)</term><term>Larve (métabolisme)</term><term>Papillons de nuit (métabolisme)</term><term>Populus (MeSH)</term><term>Quercus (MeSH)</term><term>Tube digestif (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Amino Acids, Essential</term><term>Reactive Oxygen Species</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Gastrointestinal Tract</term><term>Larva</term><term>Moths</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Acides aminés essentiels</term><term>Espèces réactives de l'oxygène</term><term>Larve</term><term>Papillons de nuit</term><term>Tube digestif</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Acer</term><term>Animals</term><term>Populus</term><term>Quercus</term><term>Trees</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Acer</term><term>Animaux</term><term>Arbres</term><term>Populus</term><term>Quercus</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The essential amino acids (EAAs) arginine, histidine, lysine, and methionine, as well as cysteine (semiessential), are believed to be susceptible to reactions with reactive oxygen species (ROS) in biological systems. The decreased availability of these EAAs could harm insect nutrition, since several of them can also be limiting for protein synthesis. However, no in vivo studies have quantified the effect of ROS in the midguts of insect herbivores on EAA composition. This study examined the association between elevated levels of ROS in the midgut fluid of Lymantria dispar caterpillars and the compositions of EAAs (protein-bound + protein-free) in their midgut fluid and frass. Contrary to expectation, the compositions of EAAs were not significantly decreased by ROS in midgut fluid ex vivo when incubated with phenolic compounds. Two in vivo comparisons of low- and high-ROS-producing leaves also showed similar results: there were no significant decreases in the compositions of EAAs in the midgut fluids and/or frass of larvae with elevated levels of ROS in their midguts. In addition, waste nitrogen excretion was not significantly increased from larvae on high-ROS treatments, as would be expected if ROS produced unbalanced EAA compositions. These results suggest that L. dispar larvae are able to tolerate elevated levels of ROS in their midguts without nutritionally significant changes in the compositions of susceptible EAAs in their food.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22961657</PMID><DateCompleted><Year>2013</Year><Month>01</Month><Day>14</Day></DateCompleted><DateRevised><Year>2012</Year><Month>10</Month><Day>16</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1520-6327</ISSN><JournalIssue CitedMedium="Internet"><Volume>81</Volume><Issue>3</Issue><PubDate><Year>2012</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Archives of insect biochemistry and physiology</Title><ISOAbbreviation>Arch Insect Biochem Physiol</ISOAbbreviation></Journal><ArticleTitle>Limited effect of reactive oxygen species on the composition of susceptible essential amino acids in the midguts of Lymantria dispar caterpillars.</ArticleTitle><Pagination><MedlinePgn>160-77</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/arch.21065</ELocationID><Abstract><AbstractText>The essential amino acids (EAAs) arginine, histidine, lysine, and methionine, as well as cysteine (semiessential), are believed to be susceptible to reactions with reactive oxygen species (ROS) in biological systems. The decreased availability of these EAAs could harm insect nutrition, since several of them can also be limiting for protein synthesis. However, no in vivo studies have quantified the effect of ROS in the midguts of insect herbivores on EAA composition. This study examined the association between elevated levels of ROS in the midgut fluid of Lymantria dispar caterpillars and the compositions of EAAs (protein-bound + protein-free) in their midgut fluid and frass. Contrary to expectation, the compositions of EAAs were not significantly decreased by ROS in midgut fluid ex vivo when incubated with phenolic compounds. Two in vivo comparisons of low- and high-ROS-producing leaves also showed similar results: there were no significant decreases in the compositions of EAAs in the midgut fluids and/or frass of larvae with elevated levels of ROS in their midguts. In addition, waste nitrogen excretion was not significantly increased from larvae on high-ROS treatments, as would be expected if ROS produced unbalanced EAA compositions. These results suggest that L. dispar larvae are able to tolerate elevated levels of ROS in their midguts without nutritionally significant changes in the compositions of susceptible EAAs in their food.</AbstractText><CopyrightInformation>© 2012 Wiley Periodicals, Inc.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Barbehenn</LastName><ForeName>Raymond V</ForeName><Initials>RV</Initials><AffiliationInfo><Affiliation>Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA. rvb@umich.edu</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Niewiadomski</LastName><ForeName>Julie</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Kochmanski</LastName><ForeName>Joseph</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Constabel</LastName><ForeName>C Peter</ForeName><Initials>CP</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>09</Month><Day>07</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Arch Insect Biochem Physiol</MedlineTA><NlmUniqueID>8501752</NlmUniqueID><ISSNLinking>0739-4462</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000601">Amino Acids, Essential</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017382">Reactive Oxygen Species</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D031002" MajorTopicYN="N">Acer</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000601" MajorTopicYN="N">Amino Acids, Essential</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D041981" MajorTopicYN="N">Gastrointestinal Tract</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007814" MajorTopicYN="N">Larva</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009036" MajorTopicYN="N">Moths</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D029963" MajorTopicYN="N">Quercus</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017382" MajorTopicYN="N">Reactive Oxygen Species</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014197" MajorTopicYN="N">Trees</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2012</Year><Month>9</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2012</Year><Month>9</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>1</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">22961657</ArticleId><ArticleId IdType="doi">10.1002/arch.21065</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Michigan</li></region></list><tree><noCountry><name sortKey="Constabel, C Peter" sort="Constabel, C Peter" uniqKey="Constabel C" first="C Peter" last="Constabel">C Peter Constabel</name><name sortKey="Kochmanski, Joseph" sort="Kochmanski, Joseph" uniqKey="Kochmanski J" first="Joseph" last="Kochmanski">Joseph Kochmanski</name><name sortKey="Niewiadomski, Julie" sort="Niewiadomski, Julie" uniqKey="Niewiadomski J" first="Julie" last="Niewiadomski">Julie Niewiadomski</name></noCountry><country name="États-Unis"><region name="Michigan"><name sortKey="Barbehenn, Raymond V" sort="Barbehenn, Raymond V" uniqKey="Barbehenn R" first="Raymond V" last="Barbehenn">Raymond V. Barbehenn</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002997 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002997 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:22961657
|texte= Limited effect of reactive oxygen species on the composition of susceptible essential amino acids in the midguts of Lymantria dispar caterpillars.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:22961657" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |